USAID-Nepal and CMDN have joined hands to determine the feasibility of using eDNA technology to increase the knowledge about the distribution and ecology of native fish biodiversity in Karnali river basins in western Nepal. The Nepal Fish Biodiversity Project (NFBP) is a two year research project, which aims to develop genetic database and introduce the latest cutting-edge technology in addressing many challenges of aquatic biodiversity study in Nepal. It is employing a scientific method of molecular level study of fish diversity, implementing eDNA metabarcoding technology to monitor the aquatic biodiversity in non-invasive manner. The information obtained through genetics is much more detailed and could possibly supplement the existing knowledge about the native ichthyology. The findings from this investigation are expected to assist in better understanding of the fish diversity available in western Nepal.